For Ice taking HTGAA class in 2024 — last updated July 2024
See HTGAA 2024 for further details about the course
Ice will be engaging with ‣
See also Ice’s Website, LinkedIn Page, Google Scholar Profile
Project idea for this class (and my career): Engineered bacterial living therapeutics to combat against antimicrobial-resistant organisms
Smaller ideas so that it is appropriate for the class time frame
1. Evaluation of Antimicrobial Resistant Genes (ARGs) Metabolic Burden
2. Evaluation of Toxicity/Burden of MS2 L-protein with non-E. coli organism
3. Cell-free synthesis of novel antimicrobial peptides for space habitat
Ice’s Bio: Ice is a Ph.D. graduate from the Molecular Engineering and Sciences program at the University of Washington. His research focuses on CRISPR gene regulation technologies development in bacteria, especially CRISPR activation, and apply it for accelerating strain engineering platform for various purposes.
Ice was initially trained as a synthetic organic chemist, then shift into being an enzyme biochemist (in Thailand!!!), and now an engineer focusing on microorganisms for making chemicals (PhD) and treat diseases (further).
Ice is an active facilitator in Engineering Biology Research Consortium (EBRC) where he serves as a board member in Students and Postdocs Association (SPA) for several years in different positions.
Ice also works with SynBio4ALL Africa to develop the Synthetic Biology educational initiative for African students. This is the reason he wants to learn more from HTGAA so that he can adapt the strategies initiated from this course to improve the curriculum for African students.
Check out this lecture by Ice focusing on laboratory techniques in Synthetic Biology: PCR and Plasmid Cloning. If you’re new to Benchling, I did a DEMO starting from slide 24 and 45:00 in the recording.
Ice discussed about CRISPR Technology and gRNA design with Homework 8 included
https://www.youtube.com/watch?v=oS7IiLaW0Nw
Ice likes to draw his bear-duck avatar in different expressions representing his experiences throughout his PhD and everyday lives.
Ice holding dCas9, his favorite biomolecular tool
Ice holding an RNA hairpin the pinnacle of his gene-regulatory tool
Ice holding a manuscript that he can’t comprehend and starts crying
Ice holding his weapons before starting the fight with microbes
Ice, overachievingly, trying to triple-wield katanas and ended up hurting himself
Ice squishing heterodimeric proteins together using his internal energy. Sometimes it work~~~