<aside> ⚠️ About your Documentation Make sure to document every step of the in-silico and lab experiments. Make sketches, screenshots, notes, drawings - anything that helps you - and others understand the experiment. Your documentation should help you - and others - to understand the topic. Don’t be afraid to add things that don’t work. Show your failures - and how you did overcome them. Your Documentation should be a description of the amazing journey you are on!
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See the Gel Art: Restriction Digests and Gel Electrophoresis protocol for details. Overview:
<aside> ⚠️ Optional for Committed Listeners with Lab Access. Mandatory for MIT/Harvard Students. Documentation due start of class Feb 18
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Perform the lab experiment you designed in Part 1 and outlined in the Gel Art: Restriction Digests and Gel Electrophoresis protocol.
<aside> ⚠️ Mandatory for MIT/Harvard Students and Committed Listeners. Due start of class Feb 18
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3.1. Choose your protein.
In recitation, we discussed that you will pick a protein for your homework that you find interesting. Which protein have you chosen and why? Using one of the tools described in recitation (NCBI, UniProt, google), obtain the protein sequence for the protein you chose.
[Example from our group homework, you may notice the particular format~~~ The example below came from UniProt]
sp|P03609|LYS_BPMS2 Lysis protein OS=Escherichia phage MS2 OX=12022 PE=2 SV=1 METRFPQQSQQTPASTNRRRPFKHEDYPCRRQQRSSTLYVLIFLAIFLSKFTNQLLLSLL EAVIRTVTTLQQLLT
3.2. Reverse Translate: Protein (amino acid) sequence to DNA (nucleotide) sequence.