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Lecture

<aside> <img src="/icons/slide_green.svg" alt="/icons/slide_green.svg" width="40px" /> Lecture slides will be shared here after the class!

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<aside> <img src="/icons/video-camera_yellow.svg" alt="/icons/video-camera_yellow.svg" width="40px" /> Class Recording

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Recitation

<aside> <img src="/icons/slide_green.svg" alt="/icons/slide_green.svg" width="40px" /> Recitation slides are posted here!

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<aside> <img src="/icons/video-camera_yellow.svg" alt="/icons/video-camera_yellow.svg" width="40px" /> Recitation Recording

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Homework Part A

<aside> <img src="/icons/exclamation-mark_orange.svg" alt="/icons/exclamation-mark_orange.svg" width="40px" /> These homework questions are based on lecture questions! Mandatory for Committed Listeners.

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Patrick Boyle’s Lecture Questions:

  1. Assume that all of the molecular biology work you'd like to do could be automated, what sort of new biological questions would you ask, or what new types of products would you make?
  2. If you could make metric tons of any protein, what would you make and what positive impact could you have?

Homework Part B

<aside> <img src="/icons/exclamation-mark_orange.svg" alt="/icons/exclamation-mark_orange.svg" width="40px" /> These homework questions are based on the Bio Production Lab! Mandatory for both Committed Listeners and MIT/Harvard students.

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<aside> <img src="/icons/push-pin_green.svg" alt="/icons/push-pin_green.svg" width="40px" /> Key Links: http://docs.google.com/document/d/15-tlrejgbbr4FMpA6rKogTjlv6qXJhFqQm7o_Ppfh-I/edit?tab=t.0#heading=h.jyt74412izch

Key Papers:

  1. Gene expression pattern analysis of a recombinant Escherichia coli strain possessing high growth and lycopene production capability when using fructose as carbon source

  2. Improvement of Biomass Yield and Recombinant Gene Expression in Escherichia coli by Using Fructose as the Primary Carbon Source </aside>

  3. Which genes when transferred into E. coli will induce the production of lycopene and beta-carotene, respectively?

  4. Why do the plasmids that are transferred into the E. coli need to contain an antibiotic resistance gene?

  5. What outcomes might we expect to see when we vary the media, presence of fructose, and temperature conditions of the overnight cultures?

  6. Generally describe what “OD600” measures and how it can be interpreted in this experiment.

  7. What are other experimental setups where we may be able to use acetone to separate cellular matter from a compound we intend to measure?

  8. Why might we want to engineer E. coli to produce lycopene and beta-carotene pigments when Erwinia herbicola naturally produces them?

Learning Module

<aside> <img src="/icons/exclamation-mark_orange.svg" alt="/icons/exclamation-mark_orange.svg" width="40px" /> These homework questions are based on the Bio Production Lab! Optional but encouraged! These questions are designed to help you start thinking more closely about DNA Design.

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Exploring Carotenoid Bioproduction

1. Getting in Touch with the Carotenoid Metabolic Pathway