SynBio Read/Write/Edit Homework

Part 0: Basics of Gel Electrophoresis

• [x] Watch all lecture and recitation videos.
  • [x] Optionally watch bootcamp

Part 1: Benchling & In-silico Gel Art

See the Gel Art: Restriction Digests and Gel Electrophoresis protocol for details. Overview:

• [x] Make a free account at benchling.com
• [x] Import the Lambda DNA.
• [x] Simulate Restriction Enzyme Digestion with the following Enzymes: EcoRI HindIII BamHI KpnI EcoRV SacI SalI

The image above represents the virtual gel electrophoresis results of a restriction digest performed on the Lambda NEB DNA sequence using different restriction enzymes. The lanes correspond to digestions with:

1. EcoRI
2. HindIII
3. BamHI
4. KpnI
5. EcoRV
6. SacI
7. SalI