Lecture

<aside> <img src="/icons/video-camera_green.svg" alt="/icons/video-camera_green.svg" width="40px" /> No lecture!

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<aside> <img src="/icons/slide_yellow.svg" alt="/icons/slide_yellow.svg" width="40px" /> No lecture!

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Recitation

<aside> <img src="/icons/video-camera_green.svg" alt="/icons/video-camera_green.svg" width="40px" /> Recitation Video will be posted here

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<aside> <img src="/icons/slide_yellow.svg" alt="/icons/slide_yellow.svg" width="40px" /> Recitation Slides: https://docs.google.com/presentation/d/1BDt1BHWNa7s3KH5coSEJfWW6wu1ZIRD4uxe4SwK353o/edit#slide=id.g20fb5ad8e35_1_0

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Homework

<aside> <img src="/icons/snippet_blue.svg" alt="/icons/snippet_blue.svg" width="40px" /> Submit your code BEFORE your lab time here: https://docs.google.com/forms/d/1AyZBo6G3XbRdq5Yr_q50Ndw6_6_FQdb8Ee-5NXJk1Ko/viewform?edit_requested=true

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To ensure you're ready for lab time, please follow these steps:

• Read the pre-lab materials to familiarize yourself with the concepts and workflow.
• Create your protocol file in the Colab notebook by writing and testing your Python routine.
• Submit your completed protocol to your TA

Students from MIT/Harvard and Global Committed Listeners will have their submissions run on available robots during the scheduled lab times!

Once complete, please sign up for a time slot with a robot (MIT/Harvard students should sign up for the robot at MIT during Lab hours), and submit your colab block via this Form.  Have this submitted AT LEAST ONE DAY before your robot time slot!

If you are having any trouble with scripting, contact us as soon as possible. Do not wait until your scheduled session or you may not be able to complete the assignment.

Post Lab Questions | Mandatory for All Students

One of the great parts about having an automated robot is being able to precisely mix, deposit, and run reactions without much intervention. This year, a greater emphasis will be placed on utilizing the Opentrons to accelerate your final projects.

For this week, we’d like for you to do the following

1. Write a description about what you intend to do with automation tools for your final project. You may include example pseudocode or Python scripts, procedures you may need to automate, 3D printed holders you may need, and more.
2. Find and describe a published paper that utilizes the Opentrons or similar automation tools to achieve novel biological applications (eg automated PACE)

While your idea doesn’t need to be set in stone, we would like to see core details of what you would automate. This is due right before class and does not need to be tested on the Opentrons for now.

Example: You are creating a custom fabric, and want to deposit art onto specific parts that need to be intertwined in odd ways. You can design a 3D printed holder to attach this fabric to it, and be able to deposit bio art on top. Check out the Opentrons 3D Printing Directory.

Mona “E.coLisas” by 2023 students Jeremy Wohlwend, Jocelyn Keyser (left) and Rodmehr Basidj (right)