OT2lab2.png

Opentrons Art

https://docs.google.com/document/d/1VR1ngrwncH4kW80PHKZDGITu4GJbDa7pCE9yCs4YdUU/edit?tab=t.0#heading=h.jyt74412izch

Objective

In this two-day lab, you'll program the Opentrons OT-2 pipetting robot to create stunning, glowing designs by depositing genetically engineered E. coli onto black agar plates. These bacteria express fluorescent proteins in vibrant colors, forming "bio-art" that comes to life under UV light. It’s your chance to turn cutting-edge biotech, with a peek into automation included, into a canvas for creativity, combining science, automation, and art into a single exciting project!

screaming-e.coli.png

Concepts You’ll Learn

This week, you will be working with the Opentrons OT-2, a liquid handling robot that’s used in various life-science laboratories on a variety of applications, and learn how to incorporate automation into synthetic biology research. This lab will require you to code a script to run on the Opentrons. You will also learn how to create agar plates, a basic tool in molecular and synthetic biology research.

Lab Preparation and Protocol

Untitled

Lab Inventory

https://docs.google.com/spreadsheets/u/1/d/e/2PACX-1vS6qUEKPOfeqp2GMVDmV5kMY2QpPWVrT4fbH6QAZyjik1PkEfeDNNn01i4geF151U8K28NJgr4ZdlxB/pubhtml?gid=549870553&single=true