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Lecture (4/1)

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<aside> <img src="/icons/video-camera_yellow.svg" alt="/icons/video-camera_yellow.svg" width="40px" /> Class Recording

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Recitation (4/2)

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Homework Part A

<aside> <img src="/icons/exclamation-mark_orange.svg" alt="/icons/exclamation-mark_orange.svg" width="40px" /> Mandatory for Committed Listeners and MIT/Harvard Students

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<aside> <img src="/icons/push-pin_green.svg" alt="/icons/push-pin_green.svg" width="40px" /> Key Links:

https://dnadots.minipcr.com/wp-content/uploads/2019/09/DNAdots-Cell-Free-Tech-final_qnoa.pdf

https://pubs.acs.org/doi/10.1021/acssynbio.3c00733

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  1. Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell free expression is more beneficial than cell production.
  2. Describe the main components of a cell-free expression system and explain the role of each component.
  3. Why is energy provision regeneration critical in cell-free systems? Describe a method you could use to ensure continuous ATP supply in your cell-free experiment.
  4. Compare prokaryotic versus eukaryotic cell-free expression systems. Choose a protein to produce in each system and explain why.
  5. How would you design a cell-free experiment to optimize the expression of a membrane protein? Discuss the challenges and how you would address them in your setup.
  6. Imagine you observe a low yield of your target protein in a cell-free system. Describe three possible reasons for this and suggest a troubleshooting strategy for each.

Homework Part B - Individual Final Project Report

<aside> <img src="/icons/exclamation-mark_orange.svg" alt="/icons/exclamation-mark_orange.svg" width="40px" /> Mandatory for Committed Listeners and MIT/Harvard Students. We’d like students to start exploring their final project in depth this week! The minimum requirement is filling out Aim 1

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<aside> <img src="/icons/push-pin_green.svg" alt="/icons/push-pin_green.svg" width="40px" /> Key Links (For MIT/Harvard): https://docs.google.com/spreadsheets/d/190gXDB_T9lq6wPN_0yLEAU66tPPJehVL1PH5dQZyFAU/edit?gid=70407915#gid=70407915

Key Due Dates (For MIT/Harvard):

<aside> 💡 See past projects here: Google Slide Deck.

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<aside> 💡 Publish a copy of this onto on your own website.

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