40643_2021_413_Figa_HTML.png

Laboratory Protocol

MIT/Harvard lab protocol

MIT students will not have a lab protocol for this week. Please check back for a revised protocol.

Global labs protocol

Cell Free System Laboratory (Global Labs).pdf

Post Lab Questions | Mandatory for All Students

  1. Use the images obtained in the lab or the ones attached and quantify fluorescence fold change between treatments. Discuss the functionality of the lacI protein in reference to this experiment a the genetic circuit used.

Cell-free Recitation - 2025.png

  1. What we should expect if the induction was made inside of a bacteria like E.coli BL21 DE3?